Detection of covalent enzyme-substrate complexes of nitrilase by ion-spray mass spectroscopy
نویسندگان
چکیده
منابع مشابه
Direct characterization of enzyme-substrate complexes by using electrosonic spray ionization mass spectrometry.
Numerous reports have cited the use of electrospray ionization (ESI) mass spectrometry to characterize intact, multiplycharged proteins and protein complexes. Herein we describe the application of a variant method, electrosonic spray ionization (ESSI), to monitor dynamic changes associated with formation of binary and ternary complexes, such as enzyme–substrate and enzyme–substrate–inhibitor sy...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1990
ISSN: 0014-5793
DOI: 10.1016/0014-5793(90)80821-y